The most commonly used plant source of Ã?²-elemene is Curcuma wenyujin Y. H. Chen\n& C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO2 extract up to\n27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil,\nin which Ã?²-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of\nÃ?²-elemene from a new sourceââ?¬â?N. damascena essential oilââ?¬â?using high performance counter-current\nchromatography HPCCC was elaborated. Furthermore, since sesquiterpens are known as potent\nantimicrobials, the need for finding new agents designed to combat multi-drug resistant strains was\naddressed and the purified target compound and the essential oil were tested for its activity against a\npanel of Gram-positive and Gram-negative bacteria, fungi, and mycobacterial strains. The application\nof the mixture of petroleum ether, acetonitrile, and acetone in the ratio 2:1.5:0.5 (v/v) in the reversed\nphase mode yielded Ã?²-elemene with high purity in 70 min. The results obtained for antimicrobial\nassay clearly indicated that N. damascena
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